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Nucleic Acids Research, 1978, Vol. 5, No. 3 771-776
© 1978

Articles

Characterization of N6, O2' -dimethyladenosine from nuclear RNA of Novikoff hepatoma

P.F. Crain*,1, Yong C. Choi{dagger}, Harris Busch{dagger} and James A. McCloskey*,1

*Institute for Lipid Research and Departments of Biochemistry, Baylor College of Medicine Houston, TX 77025, USA {dagger}Department of Pharmacology, Baylor College of Medicine Houston, TX 77025, USA

Received December 21, 1977.

A modified nucleoside was isolated from low molecular weight nuclear RNA of Novikoff hepatoma, the nucleotide sequence of which was reported earlier. The structure of this novel nucleoside was shown to be N6,O2' -dimethyladenosine (m6Am) by mass spectrometry of its trimethylsilyl derivative and by comparison with the mass spectra of N6- and O-2' monomethyl model compounds. The complete characterization was carried out using 0.04 A260 units (2 µg) of m6Am.

1Present address: Department of Biopharmaceutical Sciences, University of Utah, Salt Lake City, Utah 84112


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