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Nucleic Acids Research, 1978, Vol. 5, No. 4 1121-1137
© 1978

Articles

Mapping of the Xenopus laevis 5.8S rDNA by restriction and DNA sequencing

Paul G. Boseley, Anne Tuyns and Max L. Birnstiel

Institut für Molekularbiologie II der Universität Zürich Winterthurerstr. 266A, 8057 Zürich, Switzerland

Received January 20, 1978. The location of the 5.8S rDNA within the internal transcribed spacer has been found by restriction and sequence analysis. These analyses indicate the deletion of a dinucleotide from the known rRNA sequence. Regions to the 5' and 3' of the gene contain both uncommon sequences and palindromic structures which might provide potential control points. A secondary structure model is suggested for the 5.8S rRNA incorporating the flanking sequences.


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