Skip Navigation

This Article
Right arrow Print PDF (1186K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zwieb, C.
Right arrow Articles by Brimacombe, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zwieb, C.
Right arrow Articles by Brimacombe, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 4 1189-1206
© 1978

Articles

RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits: a method for the direct analysis of the RNA regions involved in the cross-links

Christian Zwieb and Richard Brimacombe

Max-Planck-Institut für Molekulare Genetik Abt. Wittmann, Berlin-Dahlem, GFR

Received February 28, 1978. A prerequisite for topographical studies on ribosomal sub-units involving RNA-protein cross-linking is that the cross-linking sites on the RNA should be determined. Methodology is presented which offers a solution to this problem, using as a test system 30S subunits in which protein S7 has been cross-linked to the 16S RNA by ultraviolet irradiation. The method is based on a gel separation system in the presence of a non-ionic detergent. When a ribonucleoprotein fragment containing RNA-protein cross-links is applied to this system, non-cross-linked protein is removed, and simultaneously the cross-linked RNA-protein complex is separated from non-cross-linked RNA. Oligo-nucleotide analysis of the S7-RNA complex isolated in this manner showed it to consist of a region of RNA from sections P-A of the 16S RNA. A single characteristic oligonucleotide was absent from this region, and it was tentatively concluded that this missing oligonucleotide contains the actual site of cross-linking.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
A. Branch, B. Benenfeld, B. Baroudy, F. Wells, J. Gerin, and H. Robertson
An ultraviolet-sensitive RNA structural element in a viroid-like domain of the hepatitis delta virus
Science, February 3, 1989; 243(4891): 649 - 652.
[Abstract] [PDF]

Home page
 ScienceHome page
H. Noller and C. Woese
Secondary structure of 16S ribosomal RNA
Science, April 24, 1981; 212(4493): 403 - 411.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.