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Nucleic Acids Research, 1978, Vol. 5, No. 4 1273-1287
© 1978

Articles

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs

K. Murao+, H. Ishikura+, M. Albani and H. Kersten

Institut für Physiologische Chemie Wasserturmstrasse 5, 8520 Erlangen, GFR

Received December 20, 1977.

The uridine-5-0-derivatives, 5-methoxyuridine (mo5U) and uridine-5-oxy-acetic acid (cmo5U) occupy the first position of anticodons in certain tRNA species of B. subtilis and E. coli, respectively. Here we present experimental evidence showing that both modifications are derived from a common precursor, 5-hydroxyuridine. Incompletely modified tRNAAla, tRNAThr and tRNAVal were purified from B. subtilis, and submodified tRNASer and tRNAVal from E. coli met– rel–. All five tRNAs accepted methyl groups from S-adenosylmethionine with B. subtilis extracts in vitro and mo5u was formed. In B. subtilis tRNAs the mo5u was proved to be at the specific site; in. E. coli tRNAVal the mo5u was demonstrated to be present in the oligonucleotide that comprises the anticodon. In submethylated E. coli tRNAVal, 5-hydroxyuridine was detected whereas considerable amounts of cmo5U were lacking.

+Present Address: Laboratory of Chemistry, Jichi Medical School Minamikawachi-Machi, Tochigi-Ken, 329-04 Japan K. Murao was supported by a fellowship of the Deutsche Forschungsgemeinschaft Ke 98/13


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