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Nucleic Acids Research, 1978, Vol. 5, No. 4 1381-1386
© 1978

Articles

Introduction of ribonucleic acids into cells by means of liposomes

Giorgis J. Dimitriadis

National Institute for Medical Research The Ridgeway, Mill Hill, London NW7 1AA, UK

Received February 15, 1978. A method of ultramicroinjection of nucleic acids into cultured cells by means of liposomes is described. Messenger RNA, ribosomal RNA and transfer RNA were entrapped in large unilamellar liposomes and subsequently the liposomes were fused with cells. The uptake of RNA by the cells was stimulated 6 – 8 times by our method. Possible applications of microinjection of RNA by means of liposomes are discussed.


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