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Nucleic Acids Research, 1978, Vol. 5, No. 4 1387-1402
© 1978

Articles

Cleavage of Epstein-Barr virus DNA by restriction endonucleases EcoRI, HindIII and BamI

Lars Rymo and Sven Forsblom

Department of Clinical Chemistry, University of Gothenburg, Sahlgren's Hospital S-413 45 Gothenburg, and Department of Medical Biophysics, Karolinska Institute S-104 01 Stockholm 60, Sweden

Received February 28, 1978. The cleavage of the DNAs of the B95-8 and P3HR-1 virus strains of Epstein-Barr virus by the restriction endonucleases EcoRI, HindIII and BamI was investigated using a new technique for quantitative evaluation of the fluorescence of ethidium stained DNA fragments separated on agarose gels. The results obtained with B95-8 DNA showed that in addition to the limited repetitions of nucleotide sequences observed in the EcoRI and HindIII cleavage patterns, the molecule contained a BamI fragment with a molecular mass of 2.0 megadaltons which was present in a total of about 11 copies and localized to a limited part of the DNA molecule. The same sequences were also present in the P3HR-1 DNA albeit in a lower molar ratio. P3HR-1 DNA yielded restriction enzyme cleavage patterns suggesting DNA sequence heterogeneity of P3HR-1 virus. No fragment was present in more than about 4 copies per molecule of P3HR-1 DNA. Comparison of the restriction enzyme cleavage patterns of P3HR-1 and B95-8 DNA revealed a high degree of structural homology emphasized by nucleic acid hybridization experiments with EBV complementary RNA synthesized in vitro.


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