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Nucleic Acids Research, 1978, Vol. 5, No. 5 1487-1499
© 1978

Articles

DNA-binding nonhistone proteins: DNA site reassociation

L.L. Jagodzinski, J.C. Chilton and J.S. Sevall

Department of Chemistry, Texas Tech University Lubbock, TX 79409, USA

Received December 1, 1977. The DNA-binding nonhistone proteins (NHP) have been demonstrated to fractionate the rat genome into protein-bound and unbound DNA sequences. Twenty percent of highly sheared rat DNA [~350 base pair (bp)] can be retained on membrane filters as protein complexes. When extracted from the filter and retitrated with the NHP, a 4- to 5-fold enrichment of binding sites is present in the bound DNA with few, if any, sites detected in the unbound DNA. Rat DNA restricted by EcoRI endonuclease can be fractionated by its DNA-binding NHP retention characteristics. Re-association kinetics of the bound restricted sequences indicate that 45.6% is a subset of total single-copy sequence of the rat genome and 26.9% is repetitive sequences. Cross hybridization studies indicate the repetitive sequences of the bound DNA are not enriched as much as the slow component of the rat genome. Thus a 4-fold enrichment of a subset of the rat genome has been observed via NHP-DNA interactions.


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