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Nucleic Acids Research, 1978, Vol. 5, No. 5 1515-1528
© 1978

Articles

Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells

S. Detke*, J.L. Stein+ and G.S. Stein*

*Department of Biochemistry and Molecular Biology, The University of Florida Gainesville, FL 32610, USA +Department of Immunology and Medical Microbiology, The University of Florida Gainesville, FL 32610, USA

Received January 13, 1978. Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of {alpha}-amanitin (1.0 µg/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.


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