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Nucleic Acids Research, 1978, Vol. 5, No. 5 1529-1538
© 1978

Articles

Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors

Everard M. Trip and Michael Smith

Department of Biochemistry, Faculty of Medicine, University of British Columbia Vancouver, British Columbia, V6T 1W5 Canada

Received January 26, 1978. Analytical high-pressure anion-exchange chromatography on RPC-5 has been used to study the behaviour of a good primer, d(pT-T-A-G), and a poor primer, d(pT-T-T-T-T-T) in the E. coli polynucleotide phosphorylase-catalysed reactions of dADP, dCDP, dGDP and dTDP where the primer is extended, predominantly, by one or two nucleotides. The experiments provide some generalizations for obtaining optimal yields in preparative reactions. In the course of the experiments, examples of anomalous behaviour of oligo-nucleotides on RPC-5 were encountered and these are discussed.


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