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Nucleic Acids Research, 1978, Vol. 5, No. 5 1627-1637
© 1978

Articles

RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions

Yueh-Hsiu Chein and Norman Davidson

Department of Chemistry, California Institute of Technology Pasadena, CA 91125, USA

Received February 27, 1978. Rates of formation of RNA:DNA hybrids have been measured as a function of temperature and compared to DNA:DNA duplex denaturation temperatures in 4 M sodium perchlorate, 4 M NaClO4-6 M urea, and 3 M rubidium trichioracetate solvents. The usual bell shaped curves of reaction rate versus temperature were observed. The optimal temperatures for the RNA:DNA association reaction are 5° to 12° greater than the Tm's for DNA:DNA denaturation in these solvents, just as in formamide. R-loops of {varphi}80d3ilv DNA with E. coli rRNA can be formed at high efficiency in these solvents.


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