Skip Navigation

This Article
Right arrow Print PDF (556K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Smagowicz, W.J.
Right arrow Articles by Scheit, K.H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smagowicz, W.J.
Right arrow Articles by Scheit, K.H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 6 1919-1932
© 1978

Articles

Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies

W.J. Smagowicz and K.H. Scheit

Max-Planck-Institut für Biophysikalische Chemie, Abteilung Molekulare Biologie D-3400 Göttingen-Nikolausberg, Am Fassberg, GFR

Received April 4, 1978. Ternary complexes of T7 DNA, RNA polymerase and the antibiotic rifampicin carry out the promoter specific abortive initiation when dinucleoside monophosphates were employed as primers. Primed abortive initiation, leading to synthesis of trinucleoside diphosphates, only occured with combinations of primers and substrates complementary to a promoter region of 8 base pairs centered around the origin of transcription. The steady state kinetics of three abortive initiations at T7 promoter A3 were studied in detail. The reactions appeared to be truly ordered. Affinity constants, maximal velocities and elementary step rate constants were thus obtained. The stimulation by dinucleoside monophosphate primers is brought about by positively effecting the function of the substrate site rather then by their higher affinity to the primer site of the transcriptional complex.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
I. Artsimovitch, C. Chu, A. S. Lynch, and R. Landick
A New Class of Bacterial RNA Polymerase Inhibitor Affects Nucleotide Addition
Science, October 24, 2003; 302(5645): 650 - 654.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Susa, R. Sen, and N. Shimamoto
Generality of the Branched Pathway in Transcription Initiation by Escherichia coli RNA Polymerase
J. Biol. Chem., May 3, 2002; 277(18): 15407 - 15412.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Heisler, G. Feng, D. J. Jin, C. A. Gross, and R. Landick
Amino Acid Substitutions in the Two Largest Subunits of Escherichia coli RNA Polymerase That Suppress a Defective Rho Termination Factor Affect Different Parts of the Transcription Complex
J. Biol. Chem., June 14, 1996; 271(24): 14572 - 14583.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.