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Nucleic Acids Research, 1978, Vol. 5, No. 6 1947-1954
© 1978

Articles

Binding of tRNA nucleotidyltransferase to Affi-Gel Blue: rapid purification of the enzyme and binding studies*

Murray P. Deutscher and Piotr Masiakowski

Department of Biochemistry, University of Connecticut Health Center Farmington, CT 06032, USA

Received March 28, 1978.

Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.

* This paper is number 22 in the series, "Reactions at the 3' Terminus of tRNA". The previous paper is reference 6.


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