Skip Navigation

This Article
Right arrow Print PDF (1081K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (52)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chamberlin, M. E.
Right arrow Articles by Davidson, E. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chamberlin, M. E.
Right arrow Articles by Davidson, E. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 6 2073-2094
© 1978

Articles

Studies on nucleic acid reassociation kinetics: V. Effects of disparity in tracer and driver fragment lengths

Margaret E. Chamberlin, Glenn A. Galau, Roy J. Britten and Eric H. Davidson

Division of Biology, California Institute of Technology Pasadena, CA 91125, USA

Received March 13, 1978. Measurements are described of the kinetics of nucleic acid strand pair reassoci ation where the complementary strands are of different lengths and are present in different concentrations. Rate constants for the reaction of labelled fragments ("tracer") with excess complementary strands ("driver") were determined, both for driver fragment length greater than tracer fragment length and for the reverse case. Second order reactions and pseudo-first order reactions utilizing strand separated drivers and tracers were studied. The nucleic acids which served for this investigation were ØX174 DNA and RNA, plasmid RSF2 124 DNA and E. coli DNA. Approximate empirical expressions relating driver and tracer fragment leñ the observed rate con stants were obtained for practical use. In long tracer-short driver reactions the ob served rate constant for the tracer reaction increases proportionately with tracer length. In long driver-short tracer reactions the rate of tracer reaction is retarded. The latter result is unexpected and appears to represent a departure from standard interpretations of the renaturation reaction.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.