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Nucleic Acids Research, 1978, Vol. 5, No. 6 2153-2163
© 1978

Articles

DNA-methylase from regenerating rat liver: purification and characterisation

D. Simon, F. Grunert, U. v. Acken, H. P. Dörining and H. Kröger

Robert Koch-Institut, Abt. Biochemie Nordufer 20, D-1000 Berlin 65, GFR

Received February 20, 1978. DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from euka- ryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)n and (dG,dC)n are also methylated. While SV40 DNA is almost not methylated, PM2 DNA is a good sub strate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous M.luteus DNA, but only 1 in 590 in homologous rat liver DNA. The high methylation level of M.hrteus DNA, an analysis of the methylated pyrimidine isostichs and a preliminary dinucleotide analysis suggest that all the CpGs in a DNA can be methylated.


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