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Nucleic Acids Research, 1978, Vol. 5, No. 7 2345-2358
© 1978

Articles

Cross-linking of nucleosomal histones with monofunctional imidoesters

Lode Wyns*, Ignace Lasters** and Raymond Hamers

Laboratorium Algemene Biologie, Instituut voor Moleculaire Biologie V.U.B., 65 Paardenstraat, 1640 St-Genesius-Rode, Belgium

Received April 28, 1978.

Cross-linking experiments with the MONOfunctional imidoester methyl-acetimidate, in the pH range 7.0 – 8.0, on rat liver nucleosomes generate a cross-linking pattern almost identical with the one observed for much longer Bifunctional reagents (e.g. dimethylsuberimidate).

Combined cross-linking and trypsin digestion experiments suggest that all or at least the great majority of this cross-linking occurs on trypsin digestible segments (or "tails") of the histones.

The formation of oligomers over such extremely short distances and especially the observation of an H3 homodimer suggests a very close proximity of half-nucleosomes.

* Fellow supported by a Solvay grant.

** Fellow of the I.W.O.N.L. (Instituut voor de aanmoediging van wetenschappelijk onderzoek in nijverheid en landbouw).


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