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Nucleic Acids Research, 1978, Vol. 5, No. 7 2493-2500
© 1978

Articles

Denaturation maps of DNA: experimental and theoretical maps of øx174 DNA

G.I. Karataev*, V.I. Permogorov*, A.V. Vologodskii** and M.D. Frank-Kamenetskii**,+

*Institute of Genetics and Selection of Microorganisms Moscow 113545, USSR **Institute of Molecular Genetics of Academy of Sciences of USSR Moscow 123182, USSR

+To whom correspondence should be addressed

Received May 31, 1978. A formaldehyde denaturation map of the replicative form of øx174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positions on the map of equilibrium denaturation theoretically obtained earlier from the known nucleotide sequence of øx174 DNA. The sequence is also used for a calculation of the maps of smoo thed AT-content. The maxima on these maps correlate well with the peaks on the denaturation maps. To reveal the causes of a good correlation between the experimental formaldehyde and theoretical equilibrium denaturation maps, the theoretical formaldehyde denaturation maps are calculated for different conditions (temperature, formaldehyde concentration) using the detailed theory of DNA interaction with formaldehyde developed earlier.


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[Abstract] [PDF]


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