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Nucleic Acids Research, 1978, Vol. 5, No. 7 2513-2524
© 1978

Articles

Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATYr, tRNAHis, tRNAAsn and tRNAAsp

Jon R. Katze

Department of Microbiology, University of Tennessee Center for the Health Sciences Memphis, TN 38163, USA

Received April 24, 1978. An examination, using reversed-phase chromatograph and cyanogen bromide treatment, of tRNATYr, tRNAHis, tRNAAsn, and tRNAAsp from SV40-transformed mouse fibroblasts grown to different cell densities, untransformed cells grown to confluence, and mouse liver indicates that: (1) The tissue cultured mouse fibroblasts examined here are hypomodified with respect to nucleoside Q, while liver tRNA is almost completely modified with respect to Q. (2) Cell density and/or proliferative state do not present as major variables in controlling the expression of Q in the present system. (3) SV40 virus transformation is not a major variable controlling the expression of Q in the present system. The present results support previous use of cyanogen bromide effected shifts in chromatographic elution as an assay for nucleoside Q.


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