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Nucleic Acids Research, 1978, Vol. 5, No. 7 2657-2664
© 1978

Articles

On the flexibility of the boundaries between the A'-form and B'-form sections in DNA molecule

Sergei K. Zavrieva, Lyudmila E. Minchenkova, Maxim D. Frank-Kamenetskiib and Valery I. Ivanov+

Institute of Molecular Biology, Academy of Sciences of the USSR Vavilov str.32, Moscow 117312, USSR aInstitute of Physiology, Academy of Sciences of GSSR Tbilissi 380060, USSR bInstitute of Molecular Genetics, Academy of Sciences of USSR Moscow 123182, USSR

+To whom to address the correspondence

Received February 28, 1978. The degree of orientation of DNA in a flow has been studied within the interval of the B' – A' transition induced by ethanol. The orientation of the B' DNA (60–65% ethanol, v/v) and that of the A' DNA (80–82% ethanol) are nearly identical. This means that both conformations have similar persistence lengths and that there is no aggregation in the course of formation of the A' form. Within the transition range (65–78% ethanol) the orientation attains a sharp minimum which coincides with the half-transition point (73% ethanol). The cooperative character of the B' – A' transition presupposes the existence of boundaries between the alternating sections of the A' and B' conformations that may entail an increassd flexibility of the DNA molecule and a corresponding drop of orientation. Theory predicts an elliptical dependence of the number of boundaries on the proportion of the A' form. The experimental degree of orientation follows the same pattern. Quantitative evaluation shows that the flexibility of a boundary is small, so that several dozen of boundaries are required to simulate free rotation.


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