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Nucleic Acids Research, 1978, Vol. 5, No. 8 2969-2978
© 1978

Articles

Ultraviolet resonance Raman study of DNA and of its interaction with actinomycin D

L. Chinsky and P.Y. Turpin

Laboratoire Curie-Institut du Radium 11, rue P. et M. Curie, 75231 Paris Cedex 05, France

Received June 2, 1978. The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chronophore.

First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 on are presented. The main Raman bands are assigned to the convenient nucleic bases by comparison with the spectra of momonucleotides obtained under the same experimental conditions. In particular, with a 300 nm excitation, the 1582 cm–1 line is provided by adenine, while the 1492 cm–1 one is almost exclusively due to guanime. Then, the DNA-Actinomycin D complex has been studied the line enhancements and the specificity of the resonance permits the displaying of the DNA spectrum free of any contribution of Actinomycin. The interaction provides a large intensity decrease of the 1492 cm–1 line : this is a direct conse quence of the orbital overlapping of the guanine 2-aminogroup with the ring nitrogen of Actinomycin in the DNA-Actinomycin {Phi};-complex.


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