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Nucleic Acids Research, 1978, Vol. 5, No. 8 3071-3080
© 1978

Articles

Excretion of newly synthesized DNA by tonsil lymphocytes

Mária Staub and F. Antoni

1st Institute of Biochemistry, Semmelweis University Medical School 1444 Budapest, 8. Hungary

Received March 13, 1978. The main part of newly synthesized DNA is preferentially released in vitro both by non-stimulated or phytohemagglutinin-stimulated tonsil lymphocytes. Freshly isolated cells excrete DNA faster than phytohemagglutinin-stimulated ones. The acid-precipitable 3H-thymidine lost can be accounted for in the culture medium as labeled, double stranded DNA isolated on hydroxyapatite column.


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