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Nucleic Acids Research, 1978, Vol. 5, No. 9 3113-3128
© 1978

Articles

Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA

R. Wall{dagger}, M. Gilmore-Heber{dagger}, R. Higuchi+, M. Komaromy, G. Paddoc{dagger},+, J. Strommer+ and W. Salser+

Molecular Biology Institute, University of California Los Angeles, CA 90024, USA {dagger}Department of Microbiology and Immunology, School of Medicine, University of California Los Angeles, CA 90024, USA +Department of Biology, University of California Los Angeles, CA 90024, USA

Received July 14, 1978. Recombinant ENA clones have been generated from mouse myelana MOPC 21 immunoglobulin K light chain mRNA. Complementary ENA (cDNA) synthesized on K light chain mRNA by reverse transcriptase was made double stranded and inserted into the bacterial plasmid vector, pMB9. Approximately 40 tetracycline-resistant transformed colonies containing light chain mRNA sequences were identified by colony hybridization. Five of these recombinant clones were selected and characterized. Three clones contain both K light chain constant and variable region sequences. Two of these three recombinant clones have been shewn to include all of the K light chain constant and variable region coding sequences. Another of the five selected recombinant clones contain K light chain constant region sequences. The remaining characterized clone appears to be derived from sequences at the 5'-end of K light chain mRNA, possibly extending to the terminal cap structure.


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