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Nucleic Acids Research, 1978, Vol. 5, No. 9 3315-3324
© 1978

Articles

Polynucleotides. LVI1. Synthesis and properties of poly(2'-deoxy-2'-fluoroinosinic acid)

Morio Ikehara, Nobuko Kakiuchi and Toshikazu Fukui

Faculty of Pharmaceutical Sciences, Osaka University Suita, Osaka 565, Japan

Received July 10, 1978. Poly(2' -deoxy-2' -fluoroinosinic acid) [poly (If)] was synthesized by polymerization of 2' -deoxy-2' -fluoroinosine 5' -diphosphate catalyzed by Escherichia coli polynucleotide phosphorylase. Although the UV absorption properties of poly(If) closely resembled those of poly(I), thermal melting curves at Na+ concentrations of 0.15M and 0.75M suggested two ordered structures for poly(If) neutral form. CD psectra taken at 0.15M Na+ concentration showed rather large amplitudes in both a peak at 273 nm and a trough at 246 nm, suggesting rather strong vertical stacking of bases. When complexed with poly-(C), poly(If) forms a double-stranded complex, poly(If).poly-(C) which has Tm's higher by 10–20° than those of poly(If) poly(C) measured under the same conditions. The CD spectrum of this complex resembled that of poly(I).poly(C). The effect of the fluorine atom at the 2'-position on thermal stability of polynucleotides is discussed.


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