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Nucleic Acids Research, 1978, Vol. 5, No. 9 3427-3438
© 1978

Articles

Differential susceptibilities of DNA polymerases-{alpha}and -ßto polyanions

Takashi Shimada1, Michiyuki Yamada1, Masanao Miwa2, Hiroshi Nagano3 and Yoshitake Mano3

1Biochemistry Division, National Cancer Center Research Institute Tsukiji 5-1-1, Chuo-ku, Tokyo 2Virology Division, National Cancer Center Research Institute Tsukiji 5-1-1, Chuo-ku, Tokyo 3Department of Physiological chemistry and Nutrition, Faculty of Medicine, University of Tokyo Hongo 7-3-1, Bunkyo-ku, Tokyo, Japan

Received June 6, 1978. The effects of various polyanions including synthetic polynucleotides on DNA polymerasesand-{alpha} and -ß from blastulae of the sea urchin Hemicentrotus pulcherrimus and HeLa cells were studied. Only DNA polymerase-{alpha} was inhibited by polyanions, such as polyvinyl sulfate, dextran sulfate, heparin, poly(G), poly(I), poly(U) and poly(ADP-Rib). Of the various polynucleotides tested, poly(G) and poly(I) were the strongest inhibitors. Kinetic studies showed that the Ki value for poly(G) was 0.3µg/ml and that poly(G) had 20-fold higher affinity than activated DNA for the template-primer site of DNA polynierase-{alpha}. Poly(U) and poly(ADP-Rib) were also inhibitory, but they were one hundredth as inhibitory as poly(G) or poly(I). Poly(A), poly(C), poly(A).poly(U) and poly(I).poly(C) were not inhibitory to DNA polymerase-{alpha}. In contrast, DNA polymerase-ß was not affected at all by these polyanions under the same conditions.


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