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Nucleic Acids Research, 1979, Vol. 6, No. 1 305-319
© 1979

Articles

Cytidine 5'-diphosphate reductase activity in phytohemagglutinin stimulated human lymphocytes

Gerda Tyrsted and Vera Gamulin*

Department of Biochemistry C, University of Copenhagen, Panum Institute Blegdamsvej 3, 2200 Copenhagen N, Denmark

Received October 13, 1978.

The optimal conditions and the effect of deoxyribonucleoside triphosphates were determined for CDP reductase activity in PHA-stimulated lymphocytes. The enzymatic reaction showed an absolute requirement for ATP. In the absence of ATP, only dATP showed a minor stimulation of the reduction of CDP to dCDP. During transformation the CDP reductase activity reached a maximum at the same time as the four deoxyribonucleoside triphosphate pools, corresponding to mid S-phase at about 50 h after PHA addition. The DNA polymerase activity reached a maximum at 57 h.

*present address: Institute Ruder Bos{caron}kovic Laboratorij Celularnu Biokemiju IV KR 5A, Bijenicka Cesta 54, 41000 Zagreb, Yugoslavia


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