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Nucleic Acids Research, 1979, Vol. 6, No. 1 321-330
© 1979

Articles

Synthesis of low molecular weight RNA components A, C and D by polymerase II in {alpha}-amanitin-resistant hamster cells

Elisabeth Gram Jensen, Per Hellung-Laisen* and Sune Frederiksen

Department of Biochemistry B, Panum Institute, University of Copenhagen Denmark

*Correspondence: Department of Biochemistry B, Panum Institute, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark

Received October 6, 1978. In an attempt to establish which RNA polymerase catalyzes the synthesis of the low molecular weight RNA components A, C and D, Ama 1 cells (mutant Chinese hamster cells) were used in experiments with addition of {alpha}-amanitin. Ama 1 cells contain an altered RNA polymerase II which is 800 times more resistant towards inhibition by {alpha}-amanitin than the wild type enzyme. Alpha-amanitin (up to 200 µg/ml) added to these cells does not affect the synthesis of the low molecular weight RNAs A, C and D. These data together with our previous data showing that {alpha}-amanitin (0.5 – 5.0 µg/ml) preferentially inhibits the synthesis of A, C and D in normal cells indicate that RNA polymerase II catalyzes the synthesis of the low molecular weight RNA components A, C and D.


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