Skip Navigation

This Article
Right arrow Print PDF (2062K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dickson, E.
Right arrow Articles by Robertson, H. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dickson, E.
Right arrow Articles by Robertson, H. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1979, Vol. 6, No. 1 91-110
© 1979

Articles

Approaches to sequence analysis of 125I-labeled RNA

Elizabeth Dickson, Louise K. Pape and Hugh D. Robertson

The Rockefeller University, New York NY 10021, USA

Received October 26, 1978. A method is described for the initial steps of sequence analysis of RNase Tl- and pancreatic RNase-resistant oligonucleotides of RNA containing cytidylate residues labeled in vitro with 125I. in many cases an oligo-nucleotide sequence can be deduced from a consideration of (i) its relative position in the two-dimensional fingerprint (with DEAE thin layer homo-chromatographic second dimension), (ii) its electrophoretic mobility on DEAE paper at pH 1.9, and (iii) identification of its products of further enzymatic digestion by comparison with a set of marker oligonucleotides. Additional methods including analysis of oligonucleotides following chemical blocking of uridylate residues with CMCT and analysis of products of incomplete enzymatic digestion are also discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
A. Branch and H. Robertson
A replication cycle for viroids and other small infectious RNA's
Science, February 3, 1984; 223(4635): 450 - 455.
[Abstract] [PDF]

Home page
 ScienceHome page
A. D. BRANCH, H. D. ROBERTSON, C. GREER, P. GEGENHEIMER, C. PEEBLES, and J. ABELSON
Cell-Free Circularization of Viroid Progeny RNA by an RNA Ligase from Wheat Germ
Science, September 17, 1982; 217(4565): 1147 - 1149.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.