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Nucleic Acids Research, 1979, Vol. 6, No. 10 3177-3198
© 1979


Articles

Cloning of human satellite III DNA: different components are on different chromosomes

Howard J. Cooke and John Hindley

MRC Mammalian Genome Unit King's Buildings, West Mains Road, Edinburgh EH9 3JT, UK Department of Biochemistry, University of Bristol, Medical School, University Walk Bristol BS8 1TD, UK

Received May 30, 1979. Two fragments cloned from purified human satellite III DNA do not cross-react with each other. One fragment, for which a partial sequence is reported, hybridises to satellite II as well as III and is shown to originate on chromosome 1. The other cloned fragment originates from the Y chromosome. This fragment has undergone considerable changes in size when cloned in {lambda}gt WES {lambda}B. Human satellite III is shown to consist of a number of non-cross-reacting sequences which nevertheless are related by the presence of closely spaced Hin F1 sites.


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