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Nucleic Acids Research, 1979, Vol. 6, No. 2 697-713
© 1979

Articles

Long double-stranded sequences (dsRNA-B) of nuclear pre-mRNA consist of a few highly abundant classes of sequences: evidence from DNA cloning experiments

D.A. Kramerov, A.A. Grigoryan, A.P. Ryskov and G.P. Georgiev

Institute of Molecular Biology, USSR Academy of Sciences Moscow B-334, Vavilov Street, 32, USSR

Received November 20, 1978. DNA preparations from about hundred randomly selected clones containing, mouse DNA fragments were screened for the existence of sequences complementary to long double-stranded regions of pre-mRNA able to snap back after melting (dsRNA-B). Many clones containing such sequences were found. The cloned sequences can be subdivided im;o three groups: (1) those complementary to about a half (at least to 30–40%) of the total dsRNA, designated as sequences B1; (2) those complementary to a part of sequence B1; and (3) sequences complementary to about a quarter (at least to 15%) of the total dsRNA referred to as sequence B2. The size of DNA sequence complementary to dsRNA is about 400 base pairs.

Melting experiments with hybrids show that the members of B1 family are very similar if not identical, while the divergence among B2 sequences is higher, but still the number of substitutions does not exceed 9% of bases. Thus, the major part of dsRNA-B consists of a small number of highly abundant sequences as was suggested earlier on the basis of renaturation kinetics /1–3/. Sequences B1 and B2 are represented by many copies in the mouse genome and in pre-mRNA, and many of them probably do not form hairpin-like structures.


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