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Nucleic Acids Research, 1979, Vol. 6, No. 2 745-756
© 1979

Articles

Virogenic BrdU and BrdU-sensitive DNA sequences are disproportionately concentrated in the template-active chromatin of rat embryo cells

Stephen A. Schwartz

Univeisity of Chicago, Department of Pathology 950 East 59th Street, Chicago, IL 60637, USA

Received November 2, 1978. In order to characterize the molecular mechanism responsible for the BrdU-mediated activation of endogenous retrovirus from normal rat embryo cells, the previously reported selective distribution of bromo-uracil in DNA was correlated with the corresponding organization of the nucleo-protein complex in regard to nucleosome structure and template-active and -inactive chromatin. Following micrococcal nuclease digestion of chromatin labeled with either [3H]thymidine or [3H]BrdU, the amount and specific activities of the respective nucleoscmal DNA were indistinguishable. Comparable findings were obtained following direct examination of the nudease-sensitive, "spacer" DNA. However, when each chrcmatin type was fractionated into template-active and -inactive components, it was evident that [3H]bromouracil was nonrandomly more concentrated in the template-active portion in comparison to the random distribution of [3H]thymine moieties. Furthermore, it was apparent that the template-active chromatin fraction was substantially enriched in the nucleotide sequences of rat DNA known to be sensitive to the virogenic action of BrdU.


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