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Nucleic Acids Research, 1979, Vol. 6, No. 3 1203-1219
© 1979

Articles

Antibody to B. subtilis DNA polymerase III: use in enzyme purification and examination of homology among replication-specific DNA polymerases

Marjorie H. Barnes and Neal C. Brown

Department of Pharmacology, University of Massachusetts Medical School Worcester, MA 01605 USA

Received October 18, 1978. Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B.subtilis pol III.


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