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Nucleic Acids Research, 1979, Vol. 6, No. 3 953-966
© 1979

Articles

Comparison of non histone proteins selectively associated with nucleosomes with proteins released during limited DNase digestions

N. Defer+, M. Crepin++, C. Terrioux++, J. Kruh+ and F. Gros++

+Institut de Pathologie Moléculaire 24, rue du Fahbourg Saint-Jacques, 75014 Paris, Cedex 15, France ++Départment de Biologie Moléculaire, Institut Pasteur 28, rue du Docteur Roux, 75724, Paris Cedex 15, France

Received November 8, 1978. Cultured mammary cells from GR mouse were used to analyse proteins associated with the mononucleosomes and released by a short micrococcal DNase treatment of nuclei. On metrizamide density gradients, mononucleosomes appear to be heterogeneous according to their content of associated non-histone proteins. Proteins associated with the denser fraction (1.22 – 1.24 g/ml) were analysed by two dimensional electrophoresis and compared to the proteins released by DNase I treatment. All the proteins associated with mononucleosomes were also released by DNase I treatment. It could then be assumed that these proteins are associated with the active part of the genome. Additional proteins were released by micrococcal DNase treatment of the nuclei. They could be involved in a higher order organization of chromatin.


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