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Nucleic Acids Research, 1979, Vol. 6, No. 5 1817-1830
© 1979

Articles

Physical map of the seven ribosomal RNA genes of Escherichia coli

Imre Boros, Antal Kiss and Phá Venetianer

Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences 6701 Szeged, P.O.Box 521, Hungary

Received February 26, 1979. Escherichia coli DNA was digested with restriction endonucleases BamHI, PstI , EcoRI, SalI , HindIII, XhoI, BglII, SmaI, HpaI and with selected double and triple combinations of the same enzymes. The digests were electrophoresed and hybridized with 32p-labelled ribosomal RNA by using the Southern blotting technique. The resulting bands could be arranged into seven groups, and it was possible to construct a unique physical map of the seven rRNA genes (operons) of the bacterial chromosome. Mapping information obtained on several transducing phages and recombinant plasmids carrying rRNA genes, and mapping data published in the literature helped to determine the final map. The results suggest that phage {lambda} daroE152 carries a "hybridn" rRNA gene which was probably formed by recombination between two different chromosomal rRNA genes.


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