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Nucleic Acids Research, 1979, Vol. 6, No. 5 1863-1868
© 1979

Articles

Genome organization of retmvinsg. III. Restriction endonuclease cleavage maps of mouse sarcoma virus doub-tranded DNA synthesized in vitro

Inder M. Verma

Tumor Virology Labratory, The Salk Institute San Diego, CA 921 12, USA

Received January 23, 1979. Genome length complementary DNA (cDNA) transcripts were synthesized in vitro by using purified virions of a cloned isolate of mouse sarcoma virus (MSV Clone 124). The cDNA transcripts were converted to double-stranded form by utilizing DNase-digested calf thymus DNA primers and E. coli DNA polymerase I. Restriction endonucleases sal I, Hind III, Hpa I, II and xba I were found to cleave the MSV double-stranded DNA once to generate two fragments, whereas restriction endonucleases Bgl I and Hae II cleaved twice to generate three fragments. Restriction endonucleases E. coli RI and Bam HI did not cleave MSV double-stranded DNA. The order of the restriction fragments was determined in relation to the 5' and 3' ends of the genomic RWA.


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