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Nucleic Acids Research, 1979, Vol. 6, No. 5 1895-1907
© 1979

Articles

Nuckotide sequence of the three major early promoters of bacteriophage T7

Ulrich Siebenlist

Harvard Biological Laboratories, Cambridge MA 02138, USA

Received December 4, 1978. I have determined the nucleotide sequences of the three major early promoters of bacteriophage T7 (Al, A2, A 3 ). The sequences confirm the two main homologies found between other known promoters for E. coil RNA polymerase (nucleoside triphosphate:RNA nucleotidyl transferase, E.C. 2.7.7.6 [EC] ). In particular, all three T7 promoters show a very good match with the –35 region homology; the A2 and A3 promoters share a 17 basepair sequence in this region. On the other hand, the match with the Pribnow Box homology is much less pronounced and different for each T7 promoter.


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