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Nucleic Acids Research, 1979, Vol. 6, No. 5 1909-1927
© 1979

Articles

Comparison of the structure and transcriptional capability of growing phase and stationary yeast chromatin: a model for reversible gene activation

D. Lohr and G. lde

Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331, USA

Received October 5, 1978. We have compared the structure of intra-nuclear and isolated chromatin from logarithmically growing yeast cells to chromatin from cells which had entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern, 160 bp repeat size, with staphylococcal nuclease and similar variability in repeat sizes within the genome. DNase I produces the same ladder (<120 b) and a quite similar extended ladder (120–300 b) which shows that both chromatins have phased nucleosomes. However, the rate of DNase I digestion of growing phase is greater than in stationary. Functionally speaking, growing phase nuclei are 5–20 times as active in the rate of endogenous transcription (all three polymerases are involved). The transcriptional and LNase I susceptibility differences noted in nuclei are maintained in sucrose gradient isolated oligonucleosomes and mnonucleosoues from the two states.


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