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Nucleic Acids Research, 1979, Vol. 6, No. 5 2003-2016
© 1979

Articles

Initiation of translation with Pseudornonas aemginosm phage PP7 RNA: nucbtide sequence of the coat cistron ribosome binding site

Byron A Bassel* and Donald R. Mills

Institute of Cancer Research, College of Physicians and Surgeons, Columbia University New York, NY 10032, USA Department of Microbiology, Upstate Medical Center Syracuse, NY 13210

Received December 30, 1978. Initiation complex formation between PP7 RNA and ribosomes of Pseudomenas aeruginosa and Eacherichia coli has been investigated.The PP7 RNA fragments protected by both species of ribosome have been isolated, and their sequences have been determined. Only one binding site is available on the intact PP7 RNA strand, and this site is recognized by ribosomes of both species. The PP7 RNA binding site is approximately 38 nucleotides long. It contains two AUG sequences and a purine-rich segment near the 5'-end that is complementary to segments near the 3'-ends of the 16S ribosnmal RNA's of both P. aeruginosa and E. coli. In order to establish which of the AUG codons acts as the initiator, the H2N-terminal amino acid sequence of PP7 coat protein was determined. This sequence is compatible with the codon sequence following the second AUG codon. The extent of the reaction of PP7 RNA with E. coli ribosomes is greater than with P. aeruginosa ribosomes, but our results do not indicate a qualitative difference in the initial interaction between intact PP7 RNA and the ribosomes of either species.


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