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Nucleic Acids Research, 1979, Vol. 6, No. 6 2041-2056
© 1979

Articles

Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support

V.K. Potapov, V.P. Veiko, O.N. Koroleva and Z.A. Shabarova

Chemistry Department and A.N.Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University Moscow, USSR

Received March 29, 1979. Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polyaterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 t o 15 nucleotides long.

A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1–3 µmol per gram of support.


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