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Nucleic Acids Research, 1979, Vol. 6, No. 6 2327-2337
© 1979

Articles

1H NMR investigation of the conformational states of DNA in nucleosome core particles

Juli Feigon and David R. Kearns

Department of Chemistry, Univenity of California San Diego, La Jolla, CA 92093, USA

Received December 28, 1978. In this study 1H NMR has been used to investigate the conformational state of DNA in nucleosome core particles. The nucleosome core particles exhibit partially resolved low field (10–15 ppm) spectra due to imino protons In Watson-Crick base pairs (one resonance per GC or AT base pair). To a first approximation, the spectrum is virtually identical with that of protein-free 140 base pair DNA, and from this observation we draw two important conclusions: (i) Since the low field spectra of DNA are known to be sensitive to conformation, the conformation of DNA in the core particles is essentially the same as that of free DNA (presumably B-form), (ii) since kinks occurring at a frequency of 1 in 10 or 1 in 20 base pairs would result in a core particle spectrum different from that of free DNA we find no NMR evidence supporting either the Crick-Klug or the Sobell models for kinking DNA around the core histones. Linewidth considerations indicate that the rotational correlation time for the core particles is approximately 1.5xl0 sec, whereas the end-over-end tumbling time of the free 140 base pair DNA is 3x10–7 sec.


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