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Nucleic Acids Research, 1979, Vol. 7, No. 3 689-703
© 1979

Articles

Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks

D. Rabin and D.M. Crothers

Department of Chemistry, Yale University New Haven, CT 06520, USA

Received June 27, 1979. Aminomethyltrioxsalen (AMT), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. We have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of RNA secondary structure formation. E. coli 5S rRNA is used as a model system. We isolated and characterized a product that is derived from the stem region of 5S RNA.


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