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Nucleic Acids Research, 1979, Vol. 7, No. 4 895-908
© 1979

Articles

A new series of RNAs associated with the genome of spleen focus fonning virus (SFFV) and poly(A)-containing RNA from SFFV-infected cells

Fumio Harada and Yoji Ikawa

Virology Division, National Cancer Center Research Institute Tsukiji, Chuo-ku, Tokyo 104 and Department of Viral Oncology, Cancer Institute Kamiikebukuro, Toshima-ku, Tokyo 170, Japan

Received July 24, 1979. A series of low molecular weight RNAs (4.5 to 5.5S) as well as other 4 to 7S RNAs were dissociated from genomic RNA of spleen focus forming virus (SFFV) by heating. On two dimensional polyacrylamide gel electrophoresis, this series of RNAs gave a series of more than thirty spots. RNase T1 fingerprints of these spots were identical except for differences in 3'-terminal oligonucleotides, which were mainly due to different numbers of uridylic acid residues, larger RNA-molecules containing poly(U)sequences at their 3'-termini. This series of RNAs is also associated with poly(A)-containing nuclear and cytoplasmic RNAs from SFFV-infected cells.


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