Nucleic Acids Research, 1979, Vol. 7, No. 5 1175-1194
© 1979
Articles |
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S ß-globin mRNA precursor and mature 10 S ß-globin mRNA have identical map coordinates
Institut für Molekularbiologie I, Universität Zürich 8093 Zürich, Switzerland
Received August 13, 1979.
We have used a modification of the Berk-Sharp technique to determine that the 5' termini of the mouse 15 S ß-globin precursor and the mature mRNA have identical map coordinates. The modification involves the use of 5' (or 3') terminally labeled probes; it allows the detection of the precursor in the presence of excess nature mRNA.
*Permanent address: Department of Biochemistry, University of Kansas, Lawrence, KS 66045, USA
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