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Nucleic Acids Research, 1979, Vol. 7, No. 5 1263-1282
© 1979

Articles

Structure of plant nuclear and ribosomal DNA containing chromatin

B. Leber and V. Hemleben

Institut für Biologie II, Lehrstuhl für Genetik, Universität Tübingen Auf der Morgenstelle 28, 7400 Tübingen, GFR

Received June 1, 1979. Digestion of plant chromatin from Brassica pekinensis and Matthiola incana with staphylococcus nuclease leads to a DNA repeat of 175±8 and a core size of 140 base pairs. DNase I digestion results in multiples of 10 bases.

Ribosomal RNA genes were studied as a model system for active plant chromatin because of their great redundancy and their high transcriptional activity in growing and differentiating tissues. The actively transcribed genes were identified by nascent RNA of ribosomal origin still attached to its matrix DNA. Hybridization techniques were used to demonstrate that even transcriptionally active gene sequences are present in nuclease generated chromabin subunits. Comparison of the DNase I kinetics of chromatin digestion with the amount of ribosomal RNA genes which is available for hybridization at the given times indicated that ribosomal RNA genes are digested, but not preferentially degraded by DNase I.


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Home page
 Cold Spring Harb Symp Quant BiolHome page
P. Labhart, P. Ness, E. Banz, R. Parish, and T. Roller
Model for the Structure of the Active Nucleolar Chromatin
Cold Spring Harb Symp Quant Biol, January 1, 1983; 47(0): 557 - 564.
[Abstract] [PDF]


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