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Nucleic Acids Research, 1979, Vol. 7, No. 6 1429-1444
© 1979

Articles

A thermostable, sequence-specific restriction endonuclease from Bacillus stearothermophilus: BstPI

Thea Pugatsch*, and Hans Weber

Institut für Molekularbiologje und Biophysik, ETH 8093 Zürich, Switzerland Institut für Molelcularbiologie I, Universität Zürich 8093 Zürich, Switzerland

Received September 13, 1979.

A restriction endonuclease, BstPI, was purified from a strain of B. stearothermophilus, and its cleavage specificity was determined. The enzyme cleaves at palindromic sites of the general structure: 5' .-G-{blacktriangledown}-t-N-A-C-C- 3' 3' -C-C-A-N-T-G{blacktriangleup}G- 5' where N.N' can be any base pair. It produces phosphorylated 5'-termini which are single stranded over a length of 5 nucleotides. Ends generated by cleavage with BstPI can be rejoined by DNA li-gase.

*Present address: Department if Microbiology, University of Virginia, Charlottesville, VA 22901 USA


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