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Nucleic Acids Research, 1979, Vol. 7, No. 6 1469-1484
© 1979

Articles

Plasmid replication functions. IV. Promoters in the replication region of plasmid R6–5

Patrick M. Slocombe, Susan Ely and Kenneth N. Timmis

Max-Planck-Institut für Molekulare Genetik Ihnestr. 63-73, 1000 Berlin-West 33, GFR

Received August 24, 1979. Eight RNA polymerase binding sites have been shown to map within the EcoRI fragment E-2 (replication region RepA EcoRI fragment) of plasraid R6-5 and all but one have been shown to contain active promoters of transcription. Three of the identified promoters are located within a 2.7kb region essential for controlled, autonomous plasmid replication and may be involved in the functional expression of the three R6-5 replication determinants that have thus far been identified, namely the origin of vegetative replication, oriV, the replication control gene, cop, and the determinant of an essential, positive-acting element, designated RepA.


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