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Nucleic Acids Research, 1979, Vol. 7, No. 6 1485-1495
© 1979

Articles

Specific chemical labeling of DNA fragments

Hilbert Eshaghpour, Dieter Söll and DonaldM. Crothers

Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06520, USA

Received August 21, 1979. We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termin1. The procedure includes enzymatic addition of 4-th1ourid1ne, followed by reaction in mild non-denaturing conditions with the highly reactive {alpha}-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with ß-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution.


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