Nucleic Acids Research, 1979, Vol. 7, No. 6 1513-1523
© 1979
Articles |
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Laboratoire de Génétique Moléculaire, Institut de Recherche en Biologie Moleculaire, F-75221 Paris Cedex 05, France
+Send correspondence to, H. C. Birnboim,Atomic Energy of Canada Limited,Chalk River, Ontario K0J lJ0 (Canda).
Received August 3, 1979. A procedure for extracting plasmid DNA from bacterial cells 1s described. The method 1s simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.
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 C. H. Chen, E. C. Lin, W. T. K. Cheng, H. S. Sun, H. J. Mersmann, and S. T. Ding Abundantly expressed genes in pig adipose tissue: An expressed sequence tag approach J Anim Sci, October 1, 2006; 84(10): 2673 - 2683. [Abstract] [Full Text] [PDF]
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S. Friedmann, B. E. Alber, and G. Fuchs Properties of Succinyl-Coenzyme A:D-Citramalate Coenzyme A Transferase and Its Role in the Autotrophic 3-Hydroxypropionate Cycle of Chloroflexus aurantiacus. J. Bacteriol., September 1, 2006; 188(18): 6460 - 6468. [Abstract] [Full Text] [PDF]
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R. Rodicio, S. Koch, H.-P. Schmitz, and J. J. Heinisch KlRHO1 and KlPKC1 are essential for cell integrity signalling in Kluyveromyces lactis. Microbiology, September 1, 2006; 152(Pt 9): 2635 - 2649. [Abstract] [Full Text] [PDF]
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A. S. Haines, P. Akhtar, E. R. Stephens, K. Jones, C. M. Thomas, C. D. Perkins, J. R. Williams, M. J. Day, and J. C. Fry Plasmids from freshwater environments capable of IncQ retrotransfer are diverse and include pQKH54, a new IncP-1 subgroup archetype. Microbiology, September 1, 2006; 152(Pt 9): 2689 - 2701. [Abstract] [Full Text] [PDF]
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 E. Karisik, M. J. Ellington, R. Pike, R. E. Warren, D. M. Livermore, and N. Woodford Molecular characterization of plasmids encoding CTX-M-15 {beta}-lactamases from Escherichia coli strains in the United Kingdom J. Antimicrob. Chemother., September 1, 2006; 58(3): 665 - 668. [Abstract] [Full Text] [PDF]
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S. Farshad, R. Sheikhi, A. Japoni, E. Basiri, and A. Alborzi Characterization of Shigella Strains in Iran by Plasmid Profile Analysis and PCR Amplification of ipa Genes. J. Clin. Microbiol., August 1, 2006; 44(8): 2879 - 2883. [Abstract] [Full Text] [PDF]
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M. J. Droge, Y. L. Boersma, P. G. Braun, R. J. Buining, M. K. Julsing, K. G. A. Selles, J. M. van Dijl, and W. J. Quax Phage Display of an Intracellular Carboxylesterase of Bacillus subtilis: Comparison of Sec and Tat Pathway Export Capabilities. Appl. Envir. Microbiol., July 1, 2006; 72(7): 4589 - 4595. [Abstract] [Full Text] [PDF]
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D. Xu and J.-C. Cote Sequence Diversity of the Bacillus thuringiensis and B. cereus Sensu Lato Flagellin (H Antigen) Protein: Comparison with H Serotype Diversity. Appl. Envir. Microbiol., July 1, 2006; 72(7): 4653 - 4662. [Abstract] [Full Text] [PDF]
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G. Erauso, K. M. Stedman, H. J. G. van de Werken, W. Zillig, and J. van der Oost Two novel conjugative plasmids from a single strain of Sulfolobus Microbiology, July 1, 2006; 152(7): 1951 - 1968. [Abstract] [Full Text] [PDF]
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T. Oshima and F. Biville Functional identification of ygiP as a positive regulator of the ttdA-ttdB-ygjE operon Microbiology, July 1, 2006; 152(7): 2129 - 2135. [Abstract] [Full Text] [PDF]
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 S. Y. Seol, Y. T. Kim, Y. S. Jeong, J. Y. Oh, H. Y. Kang, D. C. Moon, J. Kim, Y. C. Lee, D. T. Cho, and J. C. Lee Molecular characterization of antimicrobial resistance in Shigella sonnei isolates in Korea. J. Med. Microbiol., July 1, 2006; 55(Pt 7): 871 - 877. [Abstract] [Full Text] [PDF]
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A. Hetzer, C. J. Daughney, and H. W. Morgan Cadmium Ion Biosorption by the Thermophilic Bacteria Geobacillus stearothermophilus and G. thermocatenulatus. Appl. Envir. Microbiol., June 1, 2006; 72(6): 4020 - 4027. [Abstract] [Full Text] [PDF]
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J. H. Wubbeler, T. Lutke-Eversloh, S. Van Trappen, P. Vandamme, and A. Steinbuchel Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid Int J Syst Evol Microbiol, June 1, 2006; 56(6): 1305 - 1310. [Abstract] [Full Text] [PDF]
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G. Torrea, V. Chenal-Francisque, A. Leclercq, and E. Carniel Efficient Tracing of Global Isolates of Yersinia pestis by Restriction Fragment Length Polymorphism Analysis Using Three Insertion Sequences as Probes. J. Clin. Microbiol., June 1, 2006; 44(6): 2084 - 2092. [Abstract] [Full Text] [PDF]
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S. M. Kwong, R. A. Skurray, and N. Firth Replication Control of Staphylococcal Multiresistance Plasmid pSK41: an Antisense RNA Mediates Dual-Level Regulation of Rep Expression. J. Bacteriol., June 1, 2006; 188(12): 4404 - 4412. [Abstract] [Full Text] [PDF]
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M. Adamczyk, P. Dolowy, M. Jonczyk, C. M. Thomas, and G. Jagura-Burdzy The kfrA gene is the first in a tricistronic operon required for survival of IncP-1 plasmid R751 Microbiology, June 1, 2006; 152(6): 1621 - 1637. [Abstract] [Full Text] [PDF]
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N. E. Kramer, S. A. F. T. van Hijum, J. Knol, J. Kok, and O. P. Kuipers Transcriptome Analysis Reveals Mechanisms by Which Lactococcus lactis Acquires Nisin Resistance. Antimicrob. Agents Chemother., May 1, 2006; 50(5): 1753 - 1761. [Abstract] [Full Text] [PDF]
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E. M. A. Ibrahim, M. Arenskotter, H. Luftmann, and A. Steinbuchel Identification of Poly(cis-1,4-Isoprene) Degradation Intermediates during Growth of Moderately Thermophilic Actinomycetes on Rubber and Cloning of a Functional lcp Homologue from Nocardia farcinica Strain E1. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3375 - 3382. [Abstract] [Full Text] [PDF]
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 J. Sambrook and D. W. Russell Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNA CSH Protocols, May 1, 2006; 2006(2): pdb.prot3281 - pdb.prot3281. [Full Text]
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J. Sambrook and D. W. Russell Preparation of Plasmid DNA by Alkaline Lysis with SDS: Midipreparation CSH Protocols, May 1, 2006; 2006(2): pdb.prot3901 - pdb.prot3901. [Full Text]
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J. Sambrook and D. W. Russell Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreparation CSH Protocols, May 1, 2006; 2006(2): pdb.prot4084 - pdb.prot4084. [Full Text]
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J. Sambrook and D. W. Russell Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation CSH Protocols, May 1, 2006; 2006(2): pdb.prot4090 - pdb.prot4090. [Full Text]
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A. Takaya, M. Watanabe, and T. Yamamoto Organization of tn2610 containing two transposition modules. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1143 - 1147. [Abstract] [Full Text] [PDF]
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C. N. Patel, B. W. Wortham, J. L. Lines, J. D. Fetherston, R. D. Perry, and M. A. Oliveira Polyamines are essential for the formation of plague biofilm. J. Bacteriol., April 1, 2006; 188(7): 2355 - 2363. [Abstract] [Full Text] [PDF]
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P. O Cuiv, P. Clarke, and M. O'Connell Identification and characterization of an iron-regulated gene, chtA, required for the utilization of the xenosiderophores aerobactin, rhizobactin 1021 and schizokinen by Pseudomonas aeruginosa. Microbiology, April 1, 2006; 152(Pt 4): 945 - 954. [Abstract] [Full Text] [PDF]
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M. Mikosa, M. Sochacka-Pietal, J. Baj, and D. Bartosik Identification of a transposable genomic island of Paracoccus pantotrophus DSM 11072 by its transposition to a novel entrapment vector pMMB2. Microbiology, April 1, 2006; 152(Pt 4): 1063 - 1073. [Abstract] [Full Text] [PDF]
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E. Vincze and S. Bowra Transformation of rhizobia with broad-host-range plasmids by using a freeze-thaw method. Appl. Envir. Microbiol., March 1, 2006; 72(3): 2290 - 2293. [Abstract] [Full Text] [PDF]
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A. R. Griswold, M. Jameson-Lee, and R. A. Burne Regulation and Physiologic Significance of the Agmatine Deiminase System of Streptococcus mutans UA159 J. Bacteriol., February 1, 2006; 188(3): 834 - 841. [Abstract] [Full Text] [PDF]
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Y. Elbahloul and A. Steinbuchel Engineering the Genotype of Acinetobacter sp. Strain ADP1 To Enhance Biosynthesis of Cyanophycin Appl. Envir. Microbiol., February 1, 2006; 72(2): 1410 - 1419. [Abstract] [Full Text] [PDF]
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E. T. O'Connor, A. Piekarowicz, K. V. Swanson, J. M. Griffiss, and D. C. Stein Biochemical Analysis of Lpt3, a Protein Responsible for Phosphoethanolamine Addition to Lipooligosaccharide of Pathogenic Neisseria J. Bacteriol., February 1, 2006; 188(3): 1039 - 1048. [Abstract] [Full Text] [PDF]
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F. Bardischewsky, J. Fischer, B. Holler, and C. G. Friedrich SoxV transfers electrons to the periplasm of Paracoccus pantotrophus - an essential reaction for chemotrophic sulfur oxidation Microbiology, February 1, 2006; 152(2): 465 - 472. [Abstract] [Full Text] [PDF]
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M. J. van Belkum and M. E. Stiles Characterization of the theta-type plasmid pCD3.4 from Carnobacterium divergens, and modulation of its host range by RepA mutation Microbiology, January 1, 2006; 152(1): 171 - 178. [Abstract] [Full Text] [PDF]
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