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Nucleic Acids Research, 1979, Vol. 7, No. 6 1525-1540
© 1979

Articles

Subnucleosome particles containing high mobility group proteins HMG-E and HMG-G originate from transcriptionally active chromatin

V.V. Bakayev, V.V. Schmatchenko and G.P. Georgiev

Institute of Molecular Biology, USSR Academy of Sciences Vavilov street 32, Moscow B-334, USSR

Received July 30, 1979. Subnucleosome particles SN2 and SN3 containing short DNA fragments and non-histone proteins of the high mobility group, HMG-G and HMG-B respectively, were purified from the chromatin preparations of mouse L cells partially digested with staphy-lococcal nuclease. Labeled DNAs prepared from these particles were hybridized to an excess of nuclear RNA. The binding of subnucleosomal MA was about 3-fold higher comparing to total cellular DNA fragmented to the same size. Special control experiments showed that DNA.-protein complexes present in sub-nucleosomes SN2 and SN3 preexisted in nontreated nuclei. The conclusion has been drawn that non-histone proteins HMG-G and HMG-E are associated with the DNA of transcriptionally active chromatin and are released by nuclease as subnucleosomes.


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