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Nucleic Acids Research, 1979, Vol. 7, No. 6 1649-1661
© 1979

Articles

Cross-linked informofers

V.V. Prosvimin, S. Ruzidic+ and O.P. Samarina

Institute of Molecular Biology, USSR Academy of Sciences Vavilov street 32, Moscow B-334, USSR

Received August 7, 1979.

The proteins of 3QS RNP particles containing pre-mBNA (hnRNA) were cross-linked with bifunctional reagents (dimethyl-suberimidate and dime thyl-3,3'-dithiobispropionimidate). Further treatment with 1 or 2 M NaCl dissociates all RNA from protein. However, a significant part of protein particles -informofers being cross-linked survived high salt treatment. Their sedimentation coefficients were close to those of original particles. No SNA could be detected in the informofers even after labeling the cells with a precursor for a long period of time. Sodium dodecylsulfate or urea dissociated cross-linked informofers into oligomeric polypeptides. They could be dissociated by B-mercaptoethanol treatment if a reversible cross-linking reagent had been used. The resulting polypeptides were represented by informatin. ENP particles (30S ENP or poly-particles) were reconstituted upon mixing of cross-linked informofers with pre-mRNA and removal of 2 M NaCl.

+Present address: Institute of Biology, Belgrad Yugoslavia


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