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Nucleic Acids Research, 1979, Vol. 7, No. 7 1931-1943
© 1979

Articles

New genes and promoters suggested by the DNA sequence near the end of the coliphage T7 early operon

John C. Boothroyd and Richard S. Hayward

Department of Molecular Biology, University of Edinburgh UK

Received September 28, 1979. We have employed the dideoxynucleotide chain-terminating method to deter-mine the nucleotide sequence of T7 DNA between the physical map positions 18.9% and 19.8%. The most striking features of this sequence are two perfect 21-basepair repeats, each of which appears to contain a promoter for late transcription. In each case the promoter sequence incorporates a putative translational terminator on its left (5'-side of the "sense" strand), and overlaps a potential rihosome-binding site on its right. The region probably lies immediately distal to the early operon, and may contain two short, hitherto unreported protein-coding sequences.


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