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Nucleic Acids Research, 1979, Vol. 7, No. 7 1955-1964
© 1979

Articles

The use of barium salts of protected deoxyribonudeoside-3' p-chlorophenyl phosphates for construction of oligonucleotides by the phosphotriester method: high-yield synthesis of dinucleotide blocks

G.R. Gough*, KJ. Collier{dagger}, H.L. Weith{dagger} and P.T. Gilham*

*Departments of Biological Sciences,Purdue University West Lafayette, IN 47907, USA {dagger}Department of Biochemistry,Purdue University West Lafayette, IN 47907, USA

Received September 24, 1979. A new experimental approach to the synthesis of polydeoxyribonucleotides via the phosphotriester method involves construction of oligonucleotide blocks by direct use of the easily prepared barium salts of O5'.N–protected deoxyribonucleoside–3' p–chlorophenyl phosphates aa the key monomers in condensation reactions. The procedure has been demonstrated by the rapid synthesis in high yield and purity of all sixteen fully protected dinucleotides [(MeO)2Tr]dN'–dN'°.–(CNEt), (where dN' hybull dT, dbzC, dbzA, or dibG; {ring} hybull diesterifled p–chlorophenyl phosphate). This set of molecules constitutes a "syllabary" for the preparation of defined sequence oligonucleotides.


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